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Involvement of a Golgi-resident GPI-anchored Protein in Maintenance of the Golgi Structure

机译:高尔基体驻留GPI锚定的蛋白质参与维持高尔基体的结构。

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摘要

The Golgi apparatus consists of a series of flattened cisternal membranes that are aligned in parallel to form stacks. Cytosolic-oriented Golgi-associated proteins have been identified that may coordinate or maintain the Golgi architecture. Here, we describe a novel GPI-anchored protein, Golgi-resident GPI-anchored protein (GREG) that has a brefeldin A-sensitive Golgi localization. GREG resides in the Golgi lumen as a cis-oriented homodimer, due to strong interactions between coiled-coil regions in the C termini. Dimerization of GREG as well as its Golgi localization depends on a unique tandem repeat sequence within the coiled-coil region. RNA-mediated interference of GREG expression or expression of GREG mutants reveals an essential role for GREG in maintenance of the Golgi integrity. Under these conditions, secretion of the vesicular stomatitis virus glycoprotein protein as a marker for protein transport along the secretory pathway is inhibited, suggesting a loss of Golgi function as well. These results imply the involvement of a luminal protein in Golgi structure and function.
机译:高尔基体装置由一系列扁平的胸骨膜组成,这些膜平行排列以形成堆叠。已经确定了与细胞溶质有关的高尔基体相关蛋白可以协调或维持高尔基体结构。在这里,我们描述了一种新型的GPI锚定蛋白,高尔基体GPI锚定蛋白(GREG),它具有布雷菲德菌素A敏感的高尔基体定位。由于C末端螺旋线圈区域之间的强相互作用,GREG以顺式同源二聚体形式存在于高尔基体腔中。 GREG的二聚化及其在高尔基体中的定位取决于卷曲螺旋区域内独特的串联重复序列。 RNA介导的GREG表达或GREG突变体表达的干扰揭示了GREG在维持高尔基体完整性中的重要作用。在这些条件下,水泡性口炎病毒糖蛋白蛋白作为沿分泌途径蛋白转运的标志物的分泌受到抑制,这也暗示了高尔基体功能的丧失。这些结果暗示腔蛋白参与高尔基体的结构和功能。

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